Merck, SRP0128-10UG, DNMT3a Active human

General description
DNMT3A (DNA methyltransferase 3α) is one of three (DNMT1, DNMT3a and DNMT3b) active forms of DNA methyltransferases. This gene is localized to human chromosome 2p23. DNMT3A is one of the three members of the DNMT3A family, of which DNMT3A and DNMT3B are catalytically active and DNMT3L is an inactive paralogue, which has regulatory actions in germ cells.
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Biochem/physiol Actions
DNA methyltransferases are responsible for the addition of a methyl residue to the cytosine group of CpG dinucleotides. DNMT3A (DNA methyltransferase 3α) participates in the gene expression suppression by interacting with histone modifiers, such as polycomb-group (PcG). The interaction between these two proteins is thought to be implicated in cancer pathogenesis. During embryogenesis this enzyme is responsible for the formation of genomic methylation patterns and germ line development. Polymorphisms in this gene might serve as biomarkers to determine the prognosis of gastric cancer patients. Mutations in this gene are linked with Tatton-Brown-Rahman syndrome, an overgrowth syndrome characterized by unique facial appearance, intellectual disability, and increased height.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.