[{"group": "General", "name": "Materials Required but not Supplied Recommended DNA polymerase options for PCR Q5® High-Fidelity DNA Polymerase (NEB #M0491) Q5 Hot Start Flex DNA Polymerase (NEB #M0493) Q5 Hot Start Flex 2X Master Mix (NEB #M0494) LB (Luria-Bertani) plates with appropriate antibiotic SOC Outgrowth Medium (NEB #B9020) Competent cell options for transformation NEB 5-alpha Competent E. coli (High Efficiency, NEB #C2987) NEB 10-beta Competent E. coli (High Efficiency, NEB #C3019) (Recommended for assembled products greater than 10 kb) NEB 10-beta Electrocompetent E. coli (NEB #C3020) NEB® Stable Competent E. coli (High Efficiency) (NEB #C3040) Storage Notes Store at -20°C. Thaw, vortex thoroughly before use and keep on ice.", "value": ""}]
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