NEB, E3322S, EnGen® sgRNA Synthesis Kit, S. pyogenes
The EnGen sgRNA Synthesis Kit,
Catalog Number: E3322S
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| <b>Materials Required but not Supplied</b> Target-specific DNA oligo(s) Thermocycler/37°C heat block/incubator Nuclease-free water Equipment and reagents for RNA quantitation Spin columns for RNA cleanup (e.g. NEB #T2040 ) RNase-free tubes, aerosol tips Microcentrifuge Optional Materials | Alkaline Phosphatase, Calf Instestinal (CIP) Cas9 Nuclease, S. pyogenes EnGen Spy Cas9, NLS Gels, running buffer, gel loading dye, RNA and DNA ladders, gel box |
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