| <b>Materials Required but not Supplied</b> NEBNext Multiplex Oligos for Illumina® NEBNext Multiplex Oligos options can be found at www.neb.com/oligos. Alternatively, customer supplied adaptor and primers can be used; please see information in link | https://www.neb.com/faqs/2019/03/08/can-i-use-this-nebnext-kit-with-adaptors-and-primers-from-other-vendors-than-neb SPRIselect™ Reagent Kit (Beckman Coulter®, Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881) Magnetic Rack (NEB S1515S, Alpaqua® cat. #A001322, or equivalent) 80% Ethanol (freshly prepared) Thermal cycler DNase-, RNase-free PCR strip tubes, for example TempAssure® PCR flex-free 8-tube strips (USA Scientific® #1402-4708) Bioanalyzer® or TapeStation® (Agilent® Technologies, Inc.) and associated reagents and consumables For use with NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490): 1.5 ml Microcentrifuge tube and NEB #S1506 Magnet stand or equivalent (for washing beads only) For use with NEBNext rRNA Depletion Kit v2 (Human/Mouse/Rat) (NEB #E7400) and other NEBNext RNA depletion kits that do not include beads (NEB #E7750, E7850, E7865): Agencourt® RNAClean® XP Beads (Beckman Coulter, Inc. #A63987) |
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