E6177S, NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads
Catalog Number: E6177S
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| <b>Materials Required but not Supplied</b> 80% Ethanol (freshly prepared) Nuclease-free water 0.2 ml thin wall PCR tubes NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E6609, #E7710, #E7730 or #E7600) Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent) PCR machine Vortex Microcentrifuge For NEB #E7805 only | SPRIselect® Reagent Kit (Beckman Coulter, Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881) Optional: 10 mM Tris-HCl, pH 8.0 with 10 mM NaCl (for adaptor dilution of DNA input < 100 ng) |
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