NEB, E6850S, PURExpress® Δ RF123 Kit
PURExpress
Catalog Number: E6850S
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Category
Pack Size20 units / pack
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| <b>Materials Required but not Supplied</b> General | 37°C incubator Labeling: 35S-Methionine (>1000 Ci/mmol recommended, in vitro translation grade) TCA Precipitation: TCA solutions (25%, 10%), 1 M NaOH, casamino acids, ethanol, glass fiber filters, vacuum filtration manifold SDS-PAGE: Gels and running buffer, gel apparatus, power supply, gel dryer Western Blotting: Transfer apparatus, membrane, antibodies and detection reagent Purification: Ni-NTA Agarose, Amicon Ultra- 0.5 ml, Ultracel- 100K Membrane Centrifugal Filters |
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