| <b>Materials Required but not Supplied</b> 80% Ethanol (freshly prepared) Nuclease-free Water 0.1X TE (1 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) DNA LoBind Tubes (Eppendorf #022431021) NEBNext Singleplex or Multiplex Oligos for Illumina (NEB #E7350, #E7335, #E7500, #E7600, #E6609, #E7710, or #E7730) Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent) PCR Machine For NEB #E7645 only | SPRIselect® Reagent Kit (Beckman Coulter, Inc. #B23317) or AMPure® XP Beads (Beckman Coulter, Inc. #A63881) Optional: 10 mM Tris-HCl, pH 8.0 or Tris/NaCl, pH 8.0 (10 mM Tris, 10 mM NaCl) (for adaptor dilution of DNA input ≤ 100 ng) |
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