E8015S, NEBNext® Enzymatic Methyl-seq v2 Kit
NEBNext Enzymatic Methyl-seq (EM-seq
Catalog Number: E8015S
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Category
Pack Size2 Kit
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| <b>Materials Required but not Supplied</b> NEBNext UltraShear® (M7634) or Covaris® instrument and the required tubes or other fragmentation equipment PCR strip tubes or 96-well plates Cleanup beads | SPRIselect Reagent Kit (Beckman Coulter®, Inc. #B23317), AMPure® XP beads (Beckman Coulter, Inc. #A63881) or preferred bead manufacturer Hi-Di™ Formamide (Thermo Fisher Scientific® #4401457), Formamide (Sigma #F9037-100 ml), or 0.05 N NaOH. Formamide is preferred. If using NaOH, please see FAQ associated with NEB #E8015. 80% Ethanol 10 mM Tris-HCl pH 7.5 or 8.0 or low TE (10 mM Tris-HCl pH 8.0, 0.1 mM EDTA) Nuclease-free Water Magnetic rack/stand, such as NEBNext Magnetic Separation Rack (NEB #S1515) Metal cooling block, such as Diversified Biotech® (#CHAM-1000) PCR machine |
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