NEB, M0100S, RNase R

[{"group": "General", "name": "Materials Required but not Supplied Nuclease-free Water (NEB #B1500) RNase Inhibitor, Murine (NEB #M0314) (optional) Unit Definition One unit is defined as the amount of enzyme required to convert 75 pmoles of 20-nucleotide single-stranded RNA sequence downstream of a 38-nucleotide DNA hairpin into acid soluble ribonucleotides in a total reaction volume of 20 μl in 15 minutes at 25°C. Reaction Conditions 1X RNase R Reaction Buffer Incubate at 37°C 1X RNase R Reaction Buffer 20 mM Tris-HCl 100 mM KCl 0.1 mM MgCl2 Storage Buffer 300 mM NaCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT surfactant 50% Glycerol pH 7.4 @ 25°C", "value": ""}]