NEB, M0202M, T4 DNA Ligase
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Catalog Number: M0202M
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5´ DNA termini concentration of 0.12 µM, 300- µg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer. Concentration | 400,000 cohesive end units/ml and 2,000,000 cohesive end units/ml <b>Reaction Conditions</b> 1X T4 DNA Ligase Reaction Buffer Incubate at 16°C 1X T4 DNA Ligase Reaction Buffer 50 mM Tris-HCl 10 mM MgCl2 1 mM ATP 10 mM DTT (pH 7.5 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes |
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