NEB, M0203S, T4 DNA Polymerase
T4 DNA Polymerase catalyzes the synthesis of DNA in the 5´→ 3´ direction and requires the presence of template and primer.
Catalog Number: M0203S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C (5). <b>Reaction Conditions</b> 1X NEBuffer™ r2.1 1X NEBuffer™ r2.1 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Activity in NEBuffers</b> <b>Storage Buffer</b> 100 mM KPO4 1 mM DTT 50% Glycerol pH 6.5 @ 25°C <b>Heat Inactivation</b> 75°C for 20 minutes <b>Molecular Weight</b> Theoretical | 104000 daltons <b>5' - 3' Exonuclease</b> No <b>3' - 5' Exonuclease</b> Yes <b>Strand Displacement</b> No <b>Unit Assay Conditions</b> 1X NEBuffer 2.1, 33 µM dNTPs including [ 3 H]-dTTP, 70 µg/ml denatured herring sperm DNA. <b>Error Rate</b> ~ 1x10 -6 bases |
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