| <b>Materials Required but not Supplied</b> Nuclease-free Water (NEB #B1500) RNase Inhibitor, Murine (NEB #M0314) (optional) <b>Unit Definition</b> One unit is defined as the amount of enzyme required to convert 1 nanomole of fluorescently labeled rA16 into a phosphatase-resistant form in 30 minutes at 37°C. <b>Reaction Conditions</b> 1X T4 RNA Ligase Reaction Buffer Supplement with 1 mM ATP Incubate at 25°C 1X T4 RNA Ligase Reaction Buffer 50 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.5 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 15 minutes <b>Unit Assay Conditions</b> 1X T4 RNA Ligase reaction buffer, supplemented with 1 mM ATP, is mixed with the RNA substrate (10µM of fluorescently labeled rA16 ) and varying amounts of enzyme. Incubation is at 37°C for 15 minutes (8). Concentration | 10,000 units/ml |
|---|
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
