NEB, M0205L, E. coli DNA Ligase
DNA Ligase will ligate these substrates:
Catalog Number: M0205L
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Category
Pack Size000 units / pack
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to give 50% ligation of HindIII fragments of λ DNA (5' DNA termini concentration of 0.12 μM, 300 μg/ml) in a total reaction volume of 20 μl in 30 minutes at 16°C in 1X E. coli DNA Ligase Reaction Buffer. Reaction Conditions 1X E. coli DNA Ligase Reaction Buffer Incubate at 16°C 1X E. coli DNA Ligase Reaction Buffer 30 mM Tris-HCl 4 mM MgCl2 26 µM NAD 1 mM DTT 50 µg/ml Recombinant Albumin (pH 8 @ 25°C) Usage Concentration 10,000 units/ml Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes", "value": ""}]
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