NEB, M0206L, Exonuclease III (E. coli)
Catalog Number: M0206L
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Pack Size000 units / pack
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble total nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 1 with 0.15 mM sonicated duplex [ 3 H]-DNA. Reaction Conditions 1X NEBuffer™ 1 Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) Storage Buffer 5 mM KPO4 200 mM KCl 5 mM β-ME 0.05 mM EDTA 200 µg/ml BSA 50% Glycerol pH 6.5 @ 25°C Heat Inactivation 70°C for 20 minutes Specific Activity 150,000 units/mg", "value": ""}]
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