NEB, M0207S, SP6 RNA Polymerase

[{"group": "General", "name": "Materials Required but not Supplied RNase Inhibitor, Murine (NEB #M0314) (optional) Ribonucleotide Solution Mix (NEB #N0466) Fresh DTT (optional) Nuclease-free Water (NEB #B1500) Unit Definition One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37°C. Reaction Conditions 1X RNAPol Reaction Buffer Supplement with 0.5 mM ATP, 0.5 mM GTP, 0.5 mM UTP and 0.5 mM CTP Incubate at 37°C 1X RNAPol Reaction Buffer 40 mM Tris-HCl 6 mM MgCl2 1 mM DTT 2 mM spermidine (pH 7.9 @ 25°C) Storage Buffer 50 mM Tris-HCl 100 mM NaCl 20 mM β-ME 1 mM EDTA 50% Glycerol 0.1% (w/v) Triton® X-100 pH 7.9 @ 25°C Unit Assay Conditions 1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and 1 μg DNA containing the SP6 promoter in 50 μl.", "value": ""}]