NEB, M0212S, Klenow Fragment (3´→5´ exo-)
DNA Pol I, Large (Klenow) fragment was originally derived as a proteolytic product of
Catalog Number: M0212S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 37°C. <b>Reaction Conditions</b> 1X NEBuffer™ 2 Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) <b>Storage Buffer</b> 25 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> 75°C for 20 minutes <b>Molecular Weight</b> Theoretical | 68000 daltons <b>5' - 3' Exonuclease</b> No <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> +++ <b>Unit Assay Conditions</b> 1X NEBuffer 2, 33 µM dNTPs including [ 3 H]-dTTP and 70 µg/ml denatured herring sperm DNA. <b>Error Rate</b> ~ 100x10 -6 bases |
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