NEB, M0214S, HpaII Methyltransferase
HpaII Methyltransferase recognizes the same sequence as the MspI Methyltransferase, but modifies the internal cytosine residue (C
Catalog Number: M0214S
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Pack Size10 units / pack
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by HpaII restriction endonuclease. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Supplement with 80 µM S-adenosylmethionine (SAM) Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Control Protection Assay</b> HpaII Methyltransferase is incubated with 1 µg of λ DNA in 10 μl 1X rCutSmart Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by HpaII Methyltransferase is determined by the addition of 40 μl NEBuffer 1 supplemented with 10 mM MgCl 2 and 10 units of HpaII restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels. <b>Storage Buffer</b> 50 mM Tris-HCl 150 mM NaCl 0.1 mM EDTA 5 mM TCEP 200 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C <b>Heat Inactivation</b> 65°C for 20 minutes <b>Molecular Weight</b> Theoretical | 40397 daltons |
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