NEB, M0219S, TaqI Methyltransferase
Catalog Number: M0219S
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Pack Size10 units / pack
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to protect 1 μg λ DNA in 1 hour at 65°C in a total reaction volume of 20 μl against cleavage by Taq I restriction endonuclease. <b>Reaction Conditions</b> 1X rCutSmart™ Buffer Supplement with 80 µM S-adenosylmethionine (SAM) Incubate at 65°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) <b>Control Protection Assay</b> Protection Assay Conditions | Taq I Methyltransferase is incubated with 1 µg λ DNA in 20 μl 1X rCutSmart Buffer and 80 µM S-adenosylmethionine, for 1 hour at 65°C. The extent of protection by Taq I Methyltransferase is determined by the addition of 30 μl 1X rCutSmart Buffer and 10 units of Taq I restriction endonuclease. Incubation for 15 minutes at 65°C is followed by analysis on an agarose gel. <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical: 47845 daltons |
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