NEB, M0220S, AluI Methyltransferase

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to protect 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by AluI restriction endonuclease. Reaction Conditions 1X AluI Methyltransferase Reaction Buffer Supplement with 80 µM S-adenosylmethionine (SAM) Incubate at 37°C Control Protection Assay AluI Methyltransferase is incubated with 1 µg of λ DNA in 10 μl 1X AluI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by AluI Methyltransferase is determined by the addition of 40 μl NEBuffer 1 supplemented with 10 mM MgCl 2 and 10 units of AluI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on an agarose gel. Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 65°C for 20 minutes", "value": ""}]