NEB, M0223S, BamHI Methyltransferase

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to protect 1 µg λ DNA in 1 hour at 37°C in a total reaction volume of 10 μl against cleavage by BamHI restriction endonuclease. Reaction Conditions 1X BamHI Methyltransferase Reaction Buffer Supplement with 80 µM S-adenosylmethionine (SAM) Incubate at 37°C Control Protection Assay BamHI Methyltransferase is incubated with 1 µg λ DNA in 10 μl 1X BamHI Methyltransferase Buffer, supplemented with 80 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes at 65°C. The extent of protection by BamHI Methyltransferase is determined by the addition of 40 μl NEBuffer 1 supplemented with 10 mM MgCl 2 and 10 units of BamHI restriction endonuclease. Incubation at 37°C for 30 minutes is followed by analysis on agarose gels. Storage Buffer 50 mM Tris-HCl 1 mM DTT 10 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation No", "value": ""}]