NEB, M0226S, CpG Methyltransferase (M.SssI)

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to protect 1 μg of λ DNA in a total reaction volume of 20 μl in 1 hour at 37°C against cleavage by BstUI restriction endonuclease. Reaction Conditions 1X NEBuffer™ 2 Supplement with 160 µM S-adenosylmethionine (SAM) Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Control Protection Assay A 20 μl reaction in NEBuffer 2 containing 1 μg of Lambda DNA, supplemented with 160 uM SAM and 1 unit of M.Sss I incubated for 1 hour at 37°C, followed by heat inactivation at 65C for 15 minutes, results in 95% protection from digestion with 10 units of BstU I in rCutSmart buffer incubated at 60°C for 30 minutes as determined by agarose gel electrophoresis. Storage Buffer 10 mM Tris-HCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 200 µg/ml BSA pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes", "value": ""}]