NEB, M0236L, T4 Polynucleotide Kinase (3' phosphatase minus)

[{"group": "General", "name": "Unit Definition One unit of enzyme catalyzes the phosphorylation of 20 pmol of fluorescently labeled oligo in 30 min at 37˚C. Reaction Conditions 1X T4 Polynucleotide Kinase Reaction Buffer Incubate at 37°C 1X T4 Polynucleotide Kinase Reaction Buffer 70 mM Tris-HCl 10 mM MgCl2 5 mM DTT (pH 7.6 @ 25°C) Usage Concentration 10,000 units/ml Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1 µM ATP pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 1X T4 Polynucleotide Kinase Reaction Buffer, 66 µM [γ -32 P] ATP (5 x 10 6 cpm/µmol), 0.26 mM 5´-hydroxyl-terminated salmon sperm DNA.", "value": ""}]