[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mix of a 23-mer and 17-mer RNAs in a total reaction volume of 20 μl in 30 minutes at 37°C. Reaction Conditions 1X T4 Rnl2 Reaction Buffer Incubate at 37°C 1X T4 Rnl2 Reaction Buffer 50 mM Tris-HCl 2 mM MgCl2 1 mM DTT 400 µM ATP (pH 7.5 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM KCl 35 mM (NH4)2SO4 0.1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 80°C for 5 minutes Specific Activity 40,000 units/mg Unit Assay Conditions 1X T4 Rnl2 Reaction Buffer and 0.4 μg of an equimolar mix of the 23-mer and 17-mer RNAs. After incubation at 37°C for 30 minutes, the ligated product is detected on a 15% polyacrylamide gel.", "value": ""}]
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