NEB, M0240L, Fpg
Catalog Number: M0240L
Send an RFQ.
Category
Pack Size0240 L
Loading…
| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave 10 pmol of a 34-mer oligonucleotide duplex containing a single 8-oxoguanine base paired with a cytosine in a total reaction volume of 10 μl in 1 hour at 37°C. <b>Reaction Conditions</b> 1X NEBuffer™ 1 Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) <b>Dilution for Comet Assay</b> Recommended dilution for the Comet Assay | 1:10 3 to 1:10 4 (4,5,6). For a protocol please visit: http://cometassay.com . <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 0.5 mM EDTA 200 µg/ml BSA 50% Glycerol pH 8 @ 25°C <b>Heat Inactivation</b> 60°C for 10 minutes |
|---|
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
