NEB, M0240S, Fpg
Catalog Number: M0240S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to cleave 10 pmol of a 34-mer oligonucleotide duplex containing a single 8-oxoguanine base paired with a cytosine in a total reaction volume of 10 μl in 1 hour at 37°C. <b>Reaction Conditions</b> 1X NEBuffer™ 1 Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X NEBuffer™ 1 10 mM Bis-Tris-Propane-HCl 10 mM MgCl2 1 mM DTT (pH 7 @ 25°C) <b>Dilution for Comet Assay</b> Recommended dilution for the Comet Assay | 1:10 3 to 1:10 4 (4,5,6). For a protocol please visit: http://cometassay.com . <b>Storage Buffer</b> 20 mM Tris-HCl 50 mM NaCl 0.5 mM EDTA 200 µg/ml BSA 50% Glycerol pH 8 @ 25°C <b>Heat Inactivation</b> 60°C for 10 minutes |
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