NEB, M0250L, Mung Bean Nuclease
Catalog Number: M0250L
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Category
Pack Size0250 L
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to digest 1 µg of M13mp18 single-stranded DNA to fragments less than 1 kb in length in a total reaction volume of 80 μl in 1X Mung Bean Nuclease Reaction Buffer when incubated for 15 minutes at 37°C. <b>Reaction Conditions</b> 1X Mung Bean Nuclease Reaction Buffer Incubate at 30°C 1X Mung Bean Nuclease Reaction Buffer 30 mM NaCl 50 mM sodium acetate 1 mM ZnSO4 (pH 5 @ 25°C) <b>Storage Buffer</b> 10 mM sodium acetate 0.1 mM Zinc Acetate 1 mM Cysteine 50% Glycerol 0.001% Triton® X-100 pH 5 @ 25°C <b>Molecular Weight</b> Theoretical | 39 kDa |
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