NEB, M0262L, Lambda Exonuclease
Catalog Number: M0262L
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Pack Size000 units / pack
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme required to produce 10 nmol of acid-soluble deoxyribonucleotide from double-stranded substrate in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Lambda Exonuclease Reaction Buffer with 1 μg sonicated duplex [ 3 H]-DNA. <b>Reaction Conditions</b> 1X Lambda Exonuclease Reaction Buffer Incubate at 37°C 1X Lambda Exonuclease Reaction Buffer 67 mM Glycine-KOH 2.5 mM MgCl2 50 µg/ml BSA (pH 9.4 @ 25°C) <b>Usage Concentration</b> 5,000 units/ml <b>Storage Buffer</b> 25 mM Tris-HCl 50 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C <b>Heat Inactivation</b> 75°C for 10 minutes <b>Molecular Weight</b> Theoretical | 28000 daltons <b>Specific Activity</b> 50,000 units/mg |
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