NEB, M0263S, T7 Exonuclease
Catalog Number: M0263S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [ 3 H]-DNA. Reaction Conditions 1X NEBuffer™ 4 Incubate at 25°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Activity in NEBuffers Storage Buffer 10 mM Tris-HCl 5 mM DTT 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C Heat Inactivation No", "value": ""}]
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