NEB, M0265L, Exonuclease T
Catalog Number: M0265L
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Category
Pack Size0265 L
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to release 1 nmol of single dT nucleotides from 50 pmol of Fam-labeled polythymidine substrate in 30 minutes at 25°C. Reaction Conditions 1X NEBuffer™ 4 Incubate at 25°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Activity in NEBuffers Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes", "value": ""}]
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