NEB, M0268S, Endonuclease III (Nth)

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 μl in 1 hour at 37°C in 1X Endonuclease III Reaction Buffer containing 10 pmol of fluorescently labeled oligonucleotide duplex. * An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. Reaction Conditions 1X Endonuclease III (Nth) Reaction Buffer Incubate at 37°C 1X Endonuclease III (Nth) Reaction Buffer 20 mM Tris-HCl 1 mM EDTA 1 mM DTT (pH 8 @ 25°C) Usage Concentration 10,000 units/ml Storage Buffer 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.4 Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 1X Endonuclease III (Nth) Reaction Buffer containing 10 pmol of fluorescently labeled oligonucleotide duplexes in a total reaction volume of 10 μl", "value": ""}]