NEB, M0269S, phi29 DNA Polymerase
Perfect for multiple displacement amplification
Catalog Number: M0269S
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| <b>Unit Definition</b> One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. <b>Reaction Conditions</b> 1X phi29 DNA Polymerase Reaction Buffer Supplement with Recombinant Albumin, Molecular Biology Grade Incubate at 30°C 1X phi29 DNA Polymerase Reaction Buffer 50 mM Tris-HCl 10 mM MgCl2 10 mM (NH4)2SO4 4 mM DTT (pH 7.5 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.5% Tween® 20 0.5% IGEPAL® CA-630 pH 7.4 @ 25°C <b>Heat Inactivation</b> 65°C for 10 minutes <b>Molecular Weight</b> Theoretical | 67000 daltons <b>5' - 3' Exonuclease</b> No <b>3' - 5' Exonuclease</b> Yes <b>Strand Displacement</b> ++++ <b>Unit Assay Conditions</b> 1X phi29 DNA Polymerase Reaction Buffer, 0.1 mg/ml Recombinant Albumin, 0.01 mg/ml HindIII-digested λ DNA, 0.2 µM dTTP including [ 3 H]-dTTP, 0.2 mM dGTP, 0.2 mM dATP and 0.2 mM dCTP. |
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