NEB, M0271L, Quick-Load® Taq 2X Master Mix
The industry standard
Catalog Number: M0271L
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Category
Pack Size33 units / pack
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| <b>1X Master Mix Composition</b> 10 mM Tris-HCl 0.05% Tween® 20 0.024% Orange G 0.003% Xylene Cyanol 33 units/ml Taq DNA Polymerase 50 mM KCl 1.5 mM MgCl2 0.2 mM dATP 0.2 mM dCTP 0.2 mM dGTP 0.2 mM dTTP 5% Glycerol 0.08% IGEPAL® CA-630 pH 8.6 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical | 94000 daltons <b>5' - 3' Exonuclease</b> Yes <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> No <b>Unit Assay Conditions</b> 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA. Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. |
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