NEB, M0273E, Taq DNA Polymerase with Standard Taq Buffer

Taq DNA Polymerase with Standard Taq Buffer is a thermostable enzyme system for routine polymerase chain reaction amplification. The enzyme catalyzes DNA synthesis in the 5′ to 3′ direction and is suitable for colony PCR, standard endpoint PCR and other applications where proofreading-level fidelity is not essential. The supplied detergent-free 10X Standard Taq Reaction Buffer supports conventional PCR workflows. This large-format package contains 20,000 enzyme units at 5,000 units/mL, divided into five 0.8 mL vials, together with the corresponding reaction-buffer pack. The enzyme lacks the 3′ to 5′ proofreading activity characteristic of high-fidelity DNA polymerases, so fidelity requirements should guide polymerase selection. Use primer-specific annealing temperatures, template-appropriate extension times and contamination controls for consistent amplification. The enzyme can generate products useful for analytical gels and conventional cloning strategies. Store enzyme and buffer at −20 °C. This format is intended for high-throughput routine PCR usage rather than a premixed complete PCR master mix.