NEB, M0273X, Taq DNA Polymerase with Standard Taq Buffer
This product is available in a glycerol-free format.
Catalog Number: M0273X
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| <b>Materials Required but not Supplied</b> Template DNA and associated forward and reverse primers Deoxynucleotide (dNTP) Solution Mix (NEB #N0447) Nuclease-free Water (NEB #B1500) <b>Unit Definition</b> One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. <b>Reaction Conditions</b> 1X Standard Taq Reaction Buffer Pack 1X Standard Taq Reaction Buffer Pack 10 mM Tris-HCl 50 mM KCl 1.5 mM MgCl2 (pH 8.3 @ 25°C) <b>Storage Buffer</b> 10 mM Tris-HCl 100 mM KCl 1 mM DTT 0.1 mM EDTA 0.5% Tween® 20 0.5% IGEPAL® CA-630 50% Glycerol pH 7.4 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical | 94000 daltons <b>5' - 3' Exonuclease</b> Yes <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> + <b>Unit Assay Conditions</b> 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA <b>Error Rate</b> ~ 285x10 -6 bases |
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