[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will incorporate10 nmoles of dNTP into acid insoluble material in 30 minutes at 37°C. Reaction Conditions 1X T7 DNA Polymerase Reaction Buffer Supplement with Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X T7 DNA Polymerase Reaction Buffer 20 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.5 @ 25°C) Storage Buffer 50 mM KPO4 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7 @ 25°C Heat Inactivation 75°C for 20 minutes 5' - 3' Exonuclease No 3' - 5' Exonuclease Yes Strand Displacement No Unit Assay Conditions 100 mM KCl, 20 mM Tris-HCl (pH 7.6), 6 mM MgCl 2 , 0.5 mM DTT, 0.1 mM EDTA, 50 ug/ml Recombinant Albumin, 150 μM dNTPs including [ 3 H]-dTTP and 70 µg/ml denatured herring sperm DNA. Error Rate ~ 15x10 -6 bases", "value": ""}]
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