NEB, M0275M, Bst DNA Polymerase, Large Fragment
This product is available in a glycerol-free format.
Catalog Number: M0275M
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| <b>Unit Definition</b> One unit is defined at the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C. <b>Reaction Conditions</b> 1X ThermoPol® Reaction Buffer Incubate at 65°C 1X ThermoPol® Reaction Buffer 20 mM Tris-HCl 10 mM (NH4)2SO4 10 mM KCl 2 mM MgSO4 0.1% Triton® X-100 (pH 8.8 @ 25°C) <b>Activity in NEBuffers</b> <b>Storage Buffer</b> 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.1% Triton® X-100 pH 7.1 @ 25°C <b>Heat Inactivation</b> 80°C for 20 minutes <b>Molecular Weight</b> Theoretical | 67000 daltons <b>5' - 3' Exonuclease</b> No <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> ++++ <b>Unit Assay Conditions</b> 50 mM KCl, 20 mM Tris-HCl (pH 8.8), 10 mM MgCl 2 , 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24 mer, 200 µM dATP, 200 µM dCTP, 200 µM dGTP, 100 µM dTTP including [ 3 H]-dTTP, and 100 µg/ml BSA. |
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