NEB, M0281L, Uracil Glycosylase Inhibitor (UGI)
Catalog Number: M0281L
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Category
Pack Size0281 L
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[{"group": "General", "name": "Unit Definition One unit of UGI is defined as the amount of protein required to inhibit one unit of E. coli UDG in 1 hour at 37°C in a total reaction volume of 50 μl. One unit of UDG is the amount of enzyme which will catalyze the release of 60 pmol of uracil per minute from double stranded, uracil-containing DNA. Reaction Conditions 1X UDG Reaction Buffer Incubate at 37°C 1X UDG Reaction Buffer 20 mM Tris-HCl 1 mM DTT 1 mM EDTA (pH 8 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM KCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 70°C for 10 minutes", "value": ""}]
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