NEB, M0282L, APE 1

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 20 pmol of a 34 mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 μl in 1 hour at 37°C. *An AP site is created by treating 20 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. Reaction Conditions 1X NEBuffer™ 4 Incubate at 37°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 50 mM NaCl 1 mM DTT 0.05 mM EDTA 200 µg/ml BSA 50% Glycerol pH 8 @ 25°C Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 1X NEBuffer 4 containing 20 pmol of fluorescently labled oligonucleotide duplex in a total reaction volume of 10 μl.", "value": ""}]