NEB, M0284S, Multiplex PCR 5X Master Mix
Multiplex with flexibility and minimal optimization
Catalog Number: M0284S
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Pack Size67 units / pack
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| <b>1X Master Mix Composition</b> 20 mM Tris-HCl 50 mM KCl 30 mM NH4Cl 2.5 mM MgCl2 0.3 mM dNTPs 3.2% Glycerol 0.08% IGEPAL® CA-630 0.07% Tween® 20 67 units/ml Taq DNA Polymerase pH 8.9 @ 25°C <b>Heat Inactivation</b> No <b>Molecular Weight</b> Theoretical | 94000 daltons <b>5' - 3' Exonuclease</b> Yes <b>3' - 5' Exonuclease</b> No <b>Strand Displacement</b> No <b>Unit Assay Conditions</b> 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA. Unit Definition: One unit of Taq DNA Polymerase is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. |
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