NEB, M0287S, LongAmp® Taq 2X Master Mix
Exceptional performance with long amplicons
Catalog Number: M0287S
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Category
Pack Size125 units / pack
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| <b>1X Master Mix Composition</b> 60 mM Tris-SO4 20 mM (NH4)2SO4 2 mM MgSO4 0.3 mM dNTPs 3% Glycerol 0.06% IGEPAL® CA-630 0.05% Tween® 20 125 units/ml LongAmp Taq DNA Polymerase pH 9.1 @ 25°C <b>Heat Inactivation</b> No <b>5' - 3' Exonuclease</b> Yes <b>3' - 5' Exonuclease</b> Yes <b>Strand Displacement</b> + <b>Unit Assay Conditions</b> 1X ThermoPol® Reaction Buffer, 200 µM dNTPs including [ 3 H]-dTTP and 15 nM primed M13 DNA. Reaction Conditions | 1X LongAmp Taq Master Mix, DNA template and primers in a total reaction volume of 25–50 μl. Unit Definition: One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 75°C. |
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