NEB, M0293S, Exonuclease I (E. coli)

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [ 3 H]-DNA. Reaction Conditions 1X Exonuclease I Reaction Buffer Incubate at 37°C 1X Exonuclease I Reaction Buffer 67 mM Glycine-KOH 6.7 mM MgCl2 10 mM β-ME (pH 9.5 @ 25°C) Usage Concentration 10,000 units/ml Storage Buffer 10 mM Tris-HCl 100 mM NaCl 5 mM β-ME 0.5 mM EDTA 100 µg/ml BSA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 80°C for 20 minutes", "value": ""}]