NEB, M0297L, RNase H
RNase H has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10250970.
Catalog Number: M0297L
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Category
Pack Size0297 L
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 20 picomoles of a fluorescently labelled 50 base pair RNA-DNA hybrid in a total reaction volume of 50 μl in 20 minutes at 37°C. Reaction Conditions 1X RNase H Reaction Buffer Incubate at 37°C 1X RNase H Reaction Buffer 50 mM Tris-HCl 75 mM KCl 3 mM MgCl2 10 mM DTT (pH 8.3 @ 25°C) Storage Buffer 50 mM KCl 10 mM Tris-HCl 0.1 mM EDTA 1 mM DTT 200 µg/ml Recombinant Albumin 50% Glycerol pH 7.4 @ 25°C Heat Inactivation 65°C for 20 minutes", "value": ""}]
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