NEB, M0298S, Cre Recombinase

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme necessary to produce maximal site-specific recombination of 0.25 μg pLox2+ control DNA in 30 minutes at 37°C in a total reaction volume of 50 μl. Maximal recombination is determined by agarose gel analysis and by transformation of reactions followed by selection on ampicillin plates. Reaction Conditions 1X Cre Recombinase Reaction Buffer Incubate at 37°C 1X Cre Recombinase Reaction Buffer 33 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 (pH 7.5 @ 25°C) Usage Concentration 1,000 - 15,000 units/ml Storage Buffer 15 mM Tris-HCl 250 mM NaCl 0.3 mg/ml BSA 50% Glycerol pH 8 @ 25°C Heat Inactivation 70°C for 10 minutes", "value": ""}]