NEB, M0299L, Endonuclease VIII
Catalog Number: M0299L
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Pack Size1 unit / pack
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 μl in 1 hour at 37°C in 1X Endonuclease VIII Reaction Buffer containing 10 pmol of fluorescently labeled oligonucleotide duplex. * An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. Reaction Conditions 1X Endonuclease VIII Reaction Buffer Incubate at 37°C 1X Endonuclease VIII Reaction Buffer 10 mM Tris-HCl 75 mM NaCl 1 mM EDTA (pH 8 @ 25°C) Storage Buffer 10 mM Tris-HCl 250 mM NaCl 0.1 mM EDTA 50% Glycerol pH 8 @ 25°C Heat Inactivation 75°C for 10 minutes Unit Assay Conditions 1X Endonclease VIII Reaction Buffer containing 10 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl.", "value": ""}]
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