NEB, M0301S, Topoisomerase I (E. coli)
Catalog Number: M0301S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that catalyzes the relaxation of > 95% of 0.5 μg of pUC19 RF I (negatively supercoiled) DNA in 15 minutes at 37°C in a total reaction volume of 25 μl. DNA supercoiling is assessed by agarose gel electrophoresis in the absence of ethidium bromide. Reaction Conditions 1X rCutSmart™ Buffer Incubate at 37°C 1X rCutSmart™ Buffer 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 100 µg/ml Recombinant Albumin (pH 7.9 @ 25°C) Activity in NEBuffers Storage Buffer 10 mM Tris-HCl 35 mM (NH4)2SO4 50 mM KCl 1 mM DTT 0.1 mM EDTA 50% Glycerol pH 7.5 @ 25°C Heat Inactivation 65°C for 20 minutes", "value": ""}]
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