NEB, M0302S, T7 Endonuclease I
Catalog Number: M0302S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to convert > 90% of 1 μg of supercoiled cruciform pUC(AT) to > 90% linear form in a total reaction volume of 50 μl in 1 hour at 37°C. Reaction Conditions 1X NEBuffer™ 2 Incubate at 37°C 1X NEBuffer™ 2 50 mM NaCl 10 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 20 mM Tris-HCl 200 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.15% Triton® X-100 pH 7.5 @ 25°C Heat Inactivation No", "value": ""}]
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