NEB, M0303S, DNase I (RNase-free)
Catalog Number: M0303S
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Pack Size000 units / pack
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[{"group": "General", "name": "Materials Required but not Supplied Nuclease-free Water (NEB #B1500) RNase Inhibitor, Murine (NEB #M0314) EDTA Unit Definition One unit is defined as the amount of enzyme which will completely degrade 1 µg of pBR322 DNA in 10 minutes at 37°C in DNase I Reaction Buffer. Complete degradation is defined as the reduction of the majority of DNA fragments to tetranucleotides or smaller. Reaction Conditions 1X DNase I Reaction Buffer Incubate at 37°C 1X DNase I Reaction Buffer 10 mM Tris-HCl 2.5 mM MgCl2 0.5 mM CaCl2 (pH 7.6 @ 25°C) Usage Concentration 2,000 units/ml Storage Buffer 10 mM Tris-HCl 2 mM CaCl2 50% Glycerol pH 7.6 @ 25°C Heat Inactivation 75°C for 10 minutes", "value": ""}]
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