NEB, M0304L, Endonuclease IV

[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34-mer oligonucleotide duplex containing a single AP site* in a total reaction volume of 10 μl in 1 hour at 37°C. *An AP site is created by treating 10 pmol of a 34 mer oligonucleotide duplex containing a single uracil residue with 1 unit of Uracil-DNA Glycosylase (UDG) for 2 minutes at 37°C. Reaction Conditions 1X NEBuffer™ 3 Incubate at 37°C 1X NEBuffer™ 3 100 mM NaCl 50 mM Tris-HCl 10 mM MgCl2 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 200 µg/ml BSA 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C Heat Inactivation 85°C for 20 minutes Unit Assay Conditions 1X NEBuffer 3 containing 10 pmol of fluorescently labeled oligonucleotide duplex in a total reaction volume of 10 μl.", "value": ""}]