NEB, M0305S, Endonuclease V
Catalog Number: M0305S
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[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme required to cleave 1 pmol of a 34 mer oligonucleotide duplex containing a single deoxyinosine site* in a total reaction volume of 10 μl in 1 hour at 37°C. * A deoxyinosine site is synthetically prepared with a dl in the middle of one strand of a 34 mer oligonucleotide duplex. Reaction Conditions 1X NEBuffer™ 4 Incubate at 37°C 1X NEBuffer™ 4 50 mM Potassium Acetate 20 mM Tris-acetate 10 mM Magnesium Acetate 1 mM DTT (pH 7.9 @ 25°C) Storage Buffer 10 mM Tris-HCl 250 mM NaCl 0.1 mM EDTA 50% Glycerol 0.15% Triton® X-100 1 mM DTT 200 µg/ml BSA pH 8 @ 25°C Heat Inactivation 65°C for 20 minutes Unit Assay Conditions 1X NEBuffer 4 containing 10 pmol of fluorescently labeled oligonucleotide duplex in a total volume of 10 μl.", "value": ""}]
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