NEB, M0308S, T4 PDG (T4 Endonuclease V)
Catalog Number: M0308S
Send an RFQ.
Category
Loading…
[{"group": "General", "name": "Unit Definition One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20 μl in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers. Reaction Conditions 1X T4 PDG Reaction Buffer Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade Incubate at 37°C 1X T4 PDG Reaction Buffer 100 mM NaCl 1 mM DTT 1 mM EDTA 25 mM Na2HPO4 (pH 7.2 @ 25°C) Storage Buffer 10 mM Tris-HCl 250 mM NaCl 1 mM DTT 0.1 mM EDTA 50% Glycerol 0.15% Triton® X-100 pH 7.4 @ 25°C Heat Inactivation No Unit Assay Conditions 1X T4 PDG Reaction Buffer containing 0.5 µg of UV irradiated supercoiled pUC19 DNA, supplemented with 100 µg/ml Recombinant Albumin in a 20 μl reaction.", "value": ""}]
Unless otherwise stated, the minimum order amount is USD 1,000.
For orders below USD 1,000, an additional handling fee may apply, or we may arrange local delivery / local handover where available.
All quotations are subject to final confirmation, including product availability, lead time, batch information, shipping cost, and applicable export or handling requirements.
For quotation requests, please send an RFQ from website or contact:
Please send inquiries directly to us in the “To” field. Emails sent via BCC, mass mailing, or with unrelated recipients may be automatically filtered and will not be processed.
